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Sains Malaysiana 52(11)(2023): 3045-3059
http://doi.org/10.17576/jsm-2023-5211-03
Advances in Sago Palm Research: A Comprehensive Review of Recent
Findings
(Kemajuan dalam Penyelidikan Pokok Sagu: Suatu Kajian Komprehensif
Penemuan Terkini)
FIFI HAFIZZAH PENDI1, WEI-JIE YAN1, HASNAIN
HUSSAIN1,*, HAIRUL AZMAN ROSLAN1 & NORZAINIZUL
JULAIHI2
1Centre for Sago Research, Faculty of Resource Science
and Technology, Universiti Malaysia Sarawak, 94300 Kota Samarahan, Sarawak,
Malaysia
2Land Custody and Development Authority, Level 4, 8
& 12, Wisma Satok, Jalan Satok, 93400 Kuching, Sarawak, Malaysia
Diserahkan:
3 Julai 2023/Diterima: 16 Oktober 2023
Abstract
The sago palm (Metroxylon
sagu Rottb.) is an indigenous plant in Papua New Guinea, Indonesia,
Malaysia, and Thailand. It provides substantial needs to the locals, especially
through the starch it produces in its trunk. Exhibiting remarkable
adaptability, this palm can thrive in challenging environments like swamps and
peat soils. This paper provides an overview of the molecular methods previously
utilised to decipher the genes responsible conferring the characteristics of
sago palm molecular techniques employed to investigate M. sagu molecular
properties include molecular markers, genome walking, rapid amplification of
cDNA ends (RACE), polymerase chain reaction (PCR) and sequencing. Additionally,
this article presents other molecular techniques that can be applied to M. sagu for future crop breeding.
Keywords: Gene expression; genome assembly; Metroxylon sagu; non-trunking; trunking
Abstrak
Pokok sagu (Metroxylon sagu Rottb.) adalah
tumbuhan asli di Papua New Guinea, Indonesia, Malaysia dan Thailand. Pokok sagu
ini sangat berguna kepada masyarakat tempatan dengan menyediakan keperluan
hidup terutamanya tepung sagu yang dihasilkan dalam batang tumbuhan unik ini.
Memiliki daya penyesuaian yang tinggi, pokok sagu boleh hidup pada persekitaran
yang mencabar seperti tanah paya dan tanah gambut. Kertas ini merumuskan teknik
molekul yang pernah digunakan untuk mempelajari dan mentafsir gen yang
bertanggungjawab terhadap ciri pokok sagu. Antara teknik molekul diaplikasikan
ialah penitian genom; kandungan cDNA amplifikasi pantas (RACE); tindak balas berantai polimerase
dan teknik penjujukan.
Kata kunci: Berbatang; himpunan genom; Metroxylon sagu; pengekspresan gen; tak berbatang
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*Pengarang untuk
surat-menyurat; email: hhasnain@unimas.my
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